{"id":2526,"date":"2023-06-06T11:53:28","date_gmt":"2023-06-06T03:53:28","guid":{"rendered":"https:\/\/www.bebi.ntu.edu.tw\/?p=2526"},"modified":"2023-06-06T11:53:28","modified_gmt":"2023-06-06T03:53:28","slug":"%e7%94%9f%e9%86%ab%e9%9b%bb%e8%b3%87%e6%89%80%e6%95%99%e5%b8%ab%e7%a0%94%e7%a9%b6%e4%ba%ae%e9%bb%9e-112%e5%b9%b46%e6%9c%88%e4%bb%bd%e3%80%8c%e9%bb%83%e5%bf%b5%e7%a5%96%e6%95%99%e6%8e%88%e3%80%8d-2","status":"publish","type":"post","link":"https:\/\/www.bebi.ntu.edu.tw\/?p=2526&lang=en","title":{"rendered":"\u751f\u91ab\u96fb\u8cc7\u6240\u6559\u5e2b\u7814\u7a76\u4eae\u9ede-112\u5e746\u6708\u4efd\u300c\u9ec3\u5ff5\u7956\u6559\u6388\u300d"},"content":{"rendered":"

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\u64b0\u5beb\uff1a\u5b78\u751f\u9673\u51a0\u5f6c<\/p>\n

 <\/p>\n

\u5fae\u6d41\u9053\u6280\u8853\u662f\u5728\u900f\u660e\u7684\u6676\u7247\u4e2d\u88fd\u4f5c\u5fae\u7c73\u5c3a\u5ea6\u7684\u8154\u5ba4\u6216\u901a\u9053\uff0c\u8b93\u751f\u7269\u6a23\u672c\u5728\u6676\u7247\u4e2d\u6d41\u52d5\u5b8c\u6210\u64cd\u4f5c\uff0c\u5305\u62ec\u62bd\u53d6\u3001\u5206\u96e2\u3001\u6df7\u5408\u3001\u5206\u6563\u548c\u79fb\u52d5\u7b49\u3002\u9019\u9805\u6280\u8853\u5c07\u539f\u5148\u9700\u8981\u5728\u6574\u500b\u5be6\u9a57\u5ba4\u4e2d\u4e0d\u540c\u90e8\u9580\u7684\u5de5\u4f5c\u6574\u5408\u5230\u4e00\u500b\u6676\u7247\u4e0a\uff0c\u56e0\u6b64\u7a31\u70ba\u300c\u6676\u7247\u4e0a\u7684\u5be6\u9a57\u5ba4\u300d\u3002<\/p>\n

\u9ec3\u5ff5\u7956\u6559\u6388\u57282021\u5e747\u6708\u767c\u8868\u5728Biosensors and Bioelectronics\u4e2d\u7684\u6587\u7ae0[1]\u63d0\u5230\uff0c\u5229\u7528\u5149\u5fae\u6d41\u9053\u7cfb\u7d71\u53ef\u4ee5\u9032\u884c\u6297\u83cc\u654f\u611f\u6027\u8a66\u9a57(AST)\u3002AST\u662f\u6cbb\u7642\u8840\u6db2\u611f\u67d3\u75c5\u60a3\u6642\u4f7f\u7528\u7684\u6280\u8853\uff0c\u901a\u904eAST\u6e2c\u8a66\u53ef\u4ee5\u78ba\u5b9a\u75c5\u60a3\u611f\u67d3\u7684\u7d30\u83cc\u7a2e\u985e\uff0c\u9032\u800c\u6295\u653e\u6709\u6548\u7684\u6297\u751f\u7d20\u6cbb\u7642\u3002\u7136\u800c\uff0c\u73fe\u6709\u7684AST\u6280\u8853\u9700\u8981\u8017\u8cbb\u6574\u665a\u6642\u9593\u9032\u884c\u6aa2\u6e2c\u3002\u56e0\u6b64\uff0c\u9019\u9805\u7814\u7a76\u63d0\u51fa\u4e86\u5229\u7528\u7d50\u5408\u5149\u5b78\u6aa2\u6e2c\u7684\u5fae\u6d41\u9053\u7cfb\u7d71\uff0c\u5728\u4e00\u7247\u6676\u7247\u4e0a\u7e2e\u77edAST\u6aa2\u6e2c\u6642\u9593\u70ba3.5\u5c0f\u6642\u3002<\/p>\n

\u57282022\u5e743\u6708\uff0c\u767c\u8868\u65bcLab on a Chip\u7684\u671f\u520a[2]\u4e2d\uff0c\u63d0\u51fa\u4e86\u9032\u5316\u7684\u5fae\u6d41\u9053AST\u6aa2\u6e2c\u6280\u8853\u3002\u65b0\u7684\u5fae\u6d41\u9053\u6676\u7247\u5177\u670964\u500b\u7368\u7acb\u7684\u5fae\u7c73\u5b54\u69fd\uff0c\u53ef\u4ee5\u5c07\u5f85\u6e2c\u751f\u7269\u6a23\u672c\u5206\u96e2\u5230\u5404\u500b\u5b54\u69fd\u4e26\u540c\u6642\u6e2c\u8a66\uff0c\u5f9e\u4e00\u7247\u6676\u7247\u53ea\u80fd\u6e2c\u8a66\u4e00\u7a2e\u7d30\u83cc\u9032\u4e00\u6b65\u589e\u52a0\u5230\u4e00\u7247\u6676\u7247\u53ef\u4ee5\u540c\u6642\u6e2c\u8a6664\u7a2e\u7d30\u83cc\u3002\u5176\u4e2d\u4f7f\u7528\u6297\u751f\u7d20\u6fc3\u5ea6\u68af\u5ea6\u5fae\u6d41\u9053\u6280\u8853\uff08ACGM\uff09\u5c07\u4e0d\u540c\u7684\u6297\u751f\u7d20\u7cbe\u78ba\u5730\u5206\u914d\u5230\u4e0d\u540c\u7684\u5b54\u69fd\u9032\u884c\u6aa2\u9a57\u3002\u6700\u91cd\u8981\u7684\u662f\uff0c\u9019\u7247\u6676\u7247\u53ea\u97005\u500b\u5c0f\u6642\u5c31\u53ef\u4ee5\u5b8c\u621064\u7a2e\u7d30\u83cc\u7684\u5206\u8fa8\u3002<\/p>\n

\u6700\u5f8c\uff0c\u57282023\u5e741\u6708\u767c\u8868\u65bcACS Sensors\u671f\u520a[3]\u4e0a\u7684\u7814\u7a76\u4e2d\u5c07\u5fae\u6d41\u9053\u6280\u8853\u61c9\u7528\u65bc\u8840\u6db2\u96e2\u5b50\u6e2c\u8a66\uff08Blood ion testing\uff09\uff0c\u7528\u65bc\u76e3\u6e2c\u4eba\u9ad4\u7684\u514d\u75ab\u72c0\u614b\u548c\u63d0\u4f9b\u91ab\u751f\u75c5\u60a3\u7684\u751f\u7406\u72c0\u614b\u8207\u75be\u75c5\u5224\u8b80\u3002\u9019\u7bc7\u7814\u7a76\u4e2d\u4f7f\u7528\u4e86\u96e2\u5b50\u654f\u611f\u578b\u96fb\u6676\u9ad4\u8207\u5fae\u6d41\u9053\u6676\u7247\u7d50\u5408\uff0c\u88fd\u4f5c\u51fa\u53ef\u4ee5\u5075\u6e2c\u751f\u7269\u6a23\u672c\u4e2d\u9209\u548c\u9240\u96e2\u5b50\u6fc3\u5ea6\u7684\u7cfb\u7d71\u3002<\/p>\n

\u751f\u7269\u5fae\u6d41\u9053\u6280\u8853\u4e0d\u50c5\u7bc0\u7701\u4e86\u6a23\u672c\u548c\u8a66\u5291\uff0c\u540c\u6642\u5177\u6709\u64cd\u4f5c\u9748\u6d3b\u6027\u9ad8\u3001\u53cd\u61c9\u901f\u5ea6\u5feb\u548c\u6c61\u67d3\u98a8\u96aa\u4f4e\u7b49\u512a\u52e2\u3002\u5728\u751f\u7269\u9ad4\u7684\u6aa2\u6e2c\u4e2d\uff0c\u5fae\u6d41\u9053\u6280\u8853\u7684\u61c9\u7528\u548c\u91cd\u8981\u6027\u65e5\u76ca\u589e\u52a0\uff0c\u53ef\u8aaa\u662f\u76f8\u7576\u524d\u77bb\u7684\u7814\u7a76\u4e3b\u984c\u3002<\/p>\n

\u7d9c\u4e0a\u6240\u8ff0\uff0c\u5fae\u6d41\u9053\u6280\u8853\u7d50\u5408\u5149\u5b78\u6aa2\u6e2c\u6280\u8853\u5728\u6297\u83cc\u654f\u611f\u6027\u8a66\u9a57\u548c\u8840\u6db2\u96e2\u5b50\u6e2c\u8a66\u65b9\u9762\u53d6\u5f97\u4e86\u986f\u8457\u7684\u9032\u5c55\uff0c\u7e2e\u77ed\u4e86\u6aa2\u6e2c\u6642\u9593\u4e26\u63d0\u4f9b\u4e86\u66f4\u7cbe\u78ba\u7684\u7d50\u679c\u3002\u9019\u4e9b\u5275\u65b0\u7684\u61c9\u7528\u6709\u52a9\u65bc\u6539\u5584\u91ab\u5b78\u8a3a\u65b7\u548c\u75c5\u60a3\u6cbb\u7642\u7684\u6548\u7387\uff0c\u4e26\u6709\u6f5b\u529b\u5728\u81e8\u5e8a\u91ab\u5b78\u9818\u57df\u7522\u751f\u91cd\u5927\u5f71\u97ff\u3002<\/p>\n

\"\" \"\"<\/p>\n

\u4e0a\u5716(\u5de6)\u70ba\u7b2c\u4e00\u4ee3\u7684AST\u5fae\u6d41\u9053\u6aa2\u6e2c\u7cfb\u7d71[1] (\u53f3)\u70ba\u7b2c\u4e8c\u4ee3\u7684AST\u5fae\u6d41\u9053\u7cfb\u7d71[2]\n

Microfluidic technology involves the fabrication of micron-sized chambers or channels within transparent chips, enabling the manipulation of biological samples to perform operations such as extraction, separation, mixing, dispersion, and movement. This technology integrates the functions of different departments in a conventional laboratory onto a single chip, earning it the nickname “lab-on-a-chip.”<\/p>\n

In an article published by Professor Huang Nianzu in July 2021 in Biosensors and Bioelectronics [1], it was mentioned that optical microfluidic systems can be used for antimicrobial susceptibility testing (AST). AST is a technique used in the treatment of bloodstream infections, where the testing helps identify the specific bacteria causing the infection, allowing for the administration of effective antibiotics. However, current AST techniques require overnight testing. To address this, the research proposed the use of a microfluidic system combined with optical detection to shorten the AST testing time to 3.5 hours on a single chip.<\/p>\n

In March 2022, an advanced microfluidic AST detection technique was published in the journal of the Lab on a Chip [2]. The new microfluidic chip features 64 independent micrometer-scale compartments, allowing the separation and simultaneous testing of different bacteria samples. This advancement enables a single chip to test 64 different bacterial strains simultaneously, compared to the previous limitation of testing only one strain per chip. The technique relies on the use of the Antibiotic Concentration Gradient Microfluidic (ACGM) method to accurately distribute different antibiotics to specific compartments for testing. Importantly, this chip can complete the discrimination of 64 bacterial strains in just 5 hours.<\/p>\n

Finally, in a study published in January 2023 in the ACS Sensors journal [3], microfluidic technology was applied to blood ion testing, which aims to monitor the immune status of the human body and provide physiological and disease-related information to healthcare professionals. This study utilized ion-sensitive field-effect transistors (ISFETs) in conjunction with microfluidic chips to create a system capable of detecting sodium and potassium ion concentrations in biological samples.<\/p>\n

Microfluidic technology offers advantages such as sample and reagent conservation, high flexibility in operation, fast response times, and low risk of contamination. Its application and importance in biomedical testing continue to grow, making it a highly promising research topic.<\/p>\n

In conclusion, the combination of microfluidic technology and optical detection has made significant progress in antimicrobial susceptibility testing and blood ion testing, reducing testing time and providing more accurate results. These innovative applications have the potential to improve the efficiency of medical diagnosis and patient treatment, and may have a profound impact in the field of clinical medicine.<\/p>\n

 <\/p>\n

Ref:<\/p>\n[1]\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 C.-C. Liao et al.<\/em>, “A microfluidic microwell device operated by the automated microfluidic control system for surface-enhanced Raman scattering-based antimicrobial susceptibility testing,” Biosensors and Bioelectronics, <\/em>vol. 191, p. 113483, 2021\/11\/01\/ 2021, doi: https:\/\/doi.org\/10.1016\/j.bios.2021.113483.<\/p>\n[2]\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 S.-J. Lin et al.<\/em>, “An antibiotic concentration gradient microfluidic device integrating surface-enhanced Raman spectroscopy for multiplex antimicrobial susceptibility testing,” Lab on a Chip, <\/em>10.1039\/D2LC00012A vol. 22, no. 9, pp. 1805-1814, 2022, doi: 10.1039\/D2LC00012A.<\/p>\n[3]\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 X.-W. Chen and N.-T. Huang, “Dual Ion-Selective Membrane Deposited Ion-Sensitive Field-Effect Transistor Integrating a Whole Blood Processing Microchamber for In Situ Blood Ion Testing,” ACS Sensors, <\/em>vol. 8, no. 2, pp. 904-913, 2023\/02\/24 2023, doi: 10.1021\/acssensors.2c02603.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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