\u7814\u7a76\u4e3b\u984c\uff1a\u8d85\u89e3\u6790\u6280\u8853\u4e4b\u61c9\u7528(Super-resolution architecture)-\u7d30\u80de\u4e2d\u5fc3\u7c92\u4e2dDAPs\u7684\u7d50\u69cb\u6a21\u578b
\n\u64b0\u5beb\uff1a\u5b78\u751f\u9673\u51a0\u5f6c<\/p>\n
\u4e86\u89e3\u7d30\u80de\u5206\u88c2\u7684\u904e\u7a0b\u5c0d\u65bc\u764c\u75c7\u7684\u7814\u7a76\u662f\u975e\u5e38\u91cd\u8981\u7684\u4e00\u74b0\uff0c\u7136\u800c\u7d30\u80de\u5206\u88c2\u662f\u4e00\u500b\u975e\u5e38\u8907\u96dc\u7684\u904e\u7a0b\u3002\u5728\u4e0d\u540c\u7684\u968e\u6bb5\uff0c\u90fd\u6709\u4e0d\u540c\u7684\u86cb\u767d\u8cea\u727d\u6d89\u5176\u4e2d\uff0c\u6700\u7d42\u624d\u80fd\u5b8c\u6210\u7d30\u80de\u7684\u5206\u88c2\u3002\u5728\u52d5\u7269\u7d30\u80de\u5206\u88c2\u7684\u9031\u671f\u5230G2 \u671f(\u5f8c\u671f)\u8207 M \u671f(\u7d42\u671f)\u4e4b\u9593\uff0c\u4e5f\u5c31\u662f\u7d30\u80de\u9032\u5165\u6709\u7d72\u5206\u88c2\u7684\u8f49\u6298\u9ede\u6642\uff0cDAPs(\u4e2d\u5fc3\u7c92\u9060\u7aef\u9644\u5c6c\u7269,Centriole distal appendages)\u7684\u904b\u4f5c\u626e\u6f14\u4e86\u91cd\u8981\u7684\u89d2\u8272\u3002\u5728DAPs\u4e2d\u7684\u4e0d\u540c\u86cb\u767d\u8cea\uff0c\u5206\u5225\u6709\u8457\u4e0d\u540c\u7684\u529f\u80fd\uff0c\u800c\u694a\u6771\u9716\u8001\u5e2b\u7684\u5718\u968a\u81f4\u529b\u65bc\u5229\u7528\u8d85\u89e3\u6790\u986f\u5fae\u93e1(Super-resolution microscopy)\u4f86\u89c0\u6e2cDAPs\u7684\u5de5\u4f5c\u904e\u7a0b\u4ee5\u53ca\u5efa\u7acb3D\u6a21\u578b\u3002<\/p>\n
\u694a\u8001\u5e2b\u65bc2018\u5e74\u767c\u8868\u5728\u81ea\u7136\u901a\u8a0a(Nature Communications)\u4e0a\u7684\u6587\u7ae0\uff0c\u5c31\u662f\u5229\u7528\u8d85\u89e3\u6790\u986f\u5fae\u93e1\u91cd\u5efaDAPs\u76843D\u6a21\u578b\u3002\u900f\u904e\u89c0\u5bdf\u6b63\u5728 G0\/G1\u968e\u6bb5\u7684\u52d5\u7269\u7d30\u80de\uff0c\u4f86\u5b9a\u4f4d\u6bcf\u4e00\u500b\u86cb\u767d\u548c\u5b83\u7684\u529f\u80fd\u3002\u800c\u52d5\u7269\u7d30\u80de\u5728\u6b64\u968e\u6bb5\u6642\uff0c\u4e2d\u5fc3\u7c92\u672b\u7aef\u6703\u751f\u9577\u51fa\u521d\u7d1a\u7e96\u6bdb\u4f86\u63d0\u4f9b\u591a\u9805\u7d30\u80de\u611f\u6e2c\u8207\u8a0a\u865f\u7684\u50b3\u905e\u3002\u800cDAPs\u5728\u9019\u500b\u904e\u7a0b\u7576\u4e2d\u626e\u6f14\u4e86\u7e96\u6bdb\u751f\u6210\u4ee5\u53ca\u5b9a\u4f4d\u7684\u5de5\u4f5c\u3002\u694a\u8001\u5e2b\u7684\u5718\u968a\u5728\u9019\u7576\u4e2d\u8fa8\u8b58\u51fa\u6578\u500bDAPs\u7684\u529f\u80fd\u86cb\u767d\u8cea\u4ee5\u53ca\u6247\u8449\u72c0\u76849\u91cd\u5c0d\u7a31\u7d50\u69cb\uff0c\u6210\u529f\u5229\u7528\u55ae\u5206\u5b50\u5b9a\u4f4d\u5f71\u50cf\u6280\u8853\u9084\u539f\u4e86DAPs\u76843D\u539f\u8c8c\uff0c\u5982\u5716\u4e00\u6240\u793a\u3002<\/p>\n
sDAPs(\u4e2d\u5fc3\u7c92\u6b21\u9060\u7aef\u9644\u5c6c\u7269, subdistal appendages)\u662f\u4e00\u500b\u9760\u8fd1DAPs\u7684\u53e6\u5916\u4e00\u500b\u4e2d\u5fc3\u7c92\u5143\u7d20\u3002\u694a\u8001\u5e2b\u65bc2020\u5e74\u5728eLife\u671f\u520a\u4e2d\u63d0\u51fa\u4e86\u4ee5\u5f80\u672a\u66fe\u767c\u73fe\u95dc\u65bcsDAPs\u7684\u751f\u7269\u6a5f\u5236\u3002\u4ee5\u5f80\u53ea\u767c\u73fe\u4e86sDAPs\u53ef\u4ee5\u5728\u521d\u7d1a\u7e96\u6bdb\u751f\u9577(ciliogenesis)\u6642\uff0c\u63d0\u4f9b\u7d21\u9318\u7d72\u9328\u5b9a\u5728\u5fae\u7ba1(microtubule)\u4e0a\u7684\u652f\u6490\u3002\u800c\u694a\u8001\u5e2b\u5229\u7528\u8d85\u89e3\u6790\u986f\u5fae\u93e1\u767c\u73feDAPs\u8ddfsDAPs\u6703\u7522\u751f\u8026\u5408\u7684\u73fe\u8c61\u3002DAPs\u4e2d\u7684CEP89\u86cb\u767d\u4ee5\u53casDAPs\u4e2d\u7684ODF2\u86cb\u767d\u5b58\u5728\u8457\u96d9\u5c64\u7684\u7d50\u69cb\uff0c\u6703\u900f\u904eDAPs\u8ddfsDAPs\u7684\u5b8c\u6574\u6027\u4f86\u505a\u8abf\u7bc0\uff0c\u6700\u7d42\u6703\u5f71\u97ffDAPs\u8ddfsDAPs\u5728\u4e2d\u5fc3\u7c92\u9060\u7aef\u4e0a\u7684\u8986\u84cb\u5340\u57df\uff0c\u5982\u5716\u4e8c\u6240\u793a\u3002<\/p>\n
\u7576\u6211\u5011\u80fd\u5920\u9084\u539f\u7684\u751f\u7269\u6a5f\u5236\u8d8a\u5b8c\u6574\uff0c\u5728\u91ab\u5b78\u4e0a\u4e5f\u53ef\u4ee5\u66f4\u4e86\u89e3\u767c\u75c5\u7684\u539f\u56e0\uff0c\u5229\u7528\u8d85\u89e3\u6790\u986f\u5fae\u93e1\u89c0\u5bdf\u7d30\u80de\u7684\u904b\u4f5c\uff0c\u4e0d\u50c5\u662f\u66f4\u4e86\u89e3\u4eba\u9ad4\u904b\u4f5c\u7684\u539f\u7406\uff0c\u4e5f\u662f\u70ba\u4e86\u672a\u4f86\u91ab\u5b78\u92ea\u5c55\u66f4\u5eb7\u838a\u7684\u5927\u9053\u3002<\/p>\n
<\/p>\n
Understanding the process of cell division is an important part of cancer research; however, cell division is a very complex process. Different proteins are involved in various stages, ultimately leading to the completion of cell division. The operation of Centriole Distal Appendages (DAPs) plays an important role when animal cells enter the turning point of mitosis during the cell division cycle, between G2 phase (late phase) and M phase (end phase). Different proteins in DAPs have other functions, and Dr. Yang’s team is developing super-resolution microscopy to observe the working process of DAPs and establish 3D models.<\/p>\n
Dr. Yang’s work in Nature Communications<\/em> (2018) reconstructed the 3D model of DAPs using super-resolution microscopy. By observing mammalian cells undergoing mitosis, the location and function of each protein were determined. When mammalian cells undergo mitosis, the centriole ends in the centrosome and forms spindle fibers to provide the necessary pulling force for separating cells. DAPs play a role in generating and positioning cilia during this process. Dr. Yang’s team identified several functional proteins in DAPs and the 9-fold symmetric structure of the DAPs, successfully restoring the configuration of DAPs via 3D modeling, as shown in Figure 1.<\/p>\n Subdistal appendages (sDAPs) are another centriole element near DAPs. Dr. Yang proposed a previously unknown biological mechanism of sDAPs in eLife<\/em> in 2020. Previously, it was only found that sDAPs may be associated with anchoring spindle fibers on microtubules during ciliogenesis. However, Dr. Yang discovered that DAPs and sDAPs were coupled based on super-resolution imaging studies. The CEP89 protein in DAPs and the ODF2 protein in sDAPs form a double-layer structure regulated by the integrity of DAPs and sDAPs, ultimately affecting the coverage area of DAPs and sDAPs at the end of the centrosome, as shown in Figure 2.<\/p>\n The more complete the biological mechanism we can uncover, the better we can understand the causes of disease in medicine. Observing the biological process of cells using super-resolution microscopy is not only about understanding the principles of human body function but also paving the way for a better future in medicine.<\/p>\n Ref: \u7814\u7a76\u4e3b\u984c\uff1a\u8d85\u89e3\u6790\u6280\u8853\u4e4b\u61c9\u7528(Super-resolution architecture)-\u7d30\u80de\u4e2d\u5fc3\u7c92\u4e2dDAPs […]\n","protected":false},"author":2,"featured_media":2423,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[19],"tags":[],"class_list":["post-2422","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-award"],"jetpack_featured_media_url":"https:\/\/www.bebi.ntu.edu.tw\/wp-content\/uploads\/2023\/03\/1678843457086.jpg","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=\/wp\/v2\/posts\/2422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2422"}],"version-history":[{"count":1,"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=\/wp\/v2\/posts\/2422\/revisions"}],"predecessor-version":[{"id":2424,"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=\/wp\/v2\/posts\/2422\/revisions\/2424"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=\/wp\/v2\/media\/2423"}],"wp:attachment":[{"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2422"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2422"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bebi.ntu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n[1]\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 T. T. Yang et al.<\/em>, “Super-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components,” Nature Communications, <\/em>vol. 9, no. 1, p. 2023, 2018\/05\/22 2018, doi: 10.1038\/s41467-018-04469-1.
\n[2]\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 W. M. Chong et al.<\/em>, “Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages,” eLife, <\/em>vol. 9, p. e53580, 2020\/04\/03 2020, doi: 10.7554\/eLife.53580.<\/p>\n","protected":false},"excerpt":{"rendered":"